User:Dileep D. Monie/Notebook/BBF Standards Measurement/2008/04/03

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2008-04-03

  • Analyzed flow cytometry data collected on 2008-04-02 using BD CellQuest Pro v5.2
  • Forward vs side scatter plot showing TOP10 cells (1% of events are shown) and R1 gate used for statistics

DDM TOP10-A.JPG

  • Histogram overlay showing FL1 signals (GFP) of representative samples from each group (gated on R1) and markers (M1 and M2) used for statistics

DDM overlay.JPG

  1. Purple solid: TOP10
  2. Green line: I20259
  3. Pink line: I20269
  4. Light blue line: I20270
  5. Orange line: I20268
  6. Dark blue line: I20260
  • Calculated geometric means of fluorescence intensities (Isample,MX) for triplicate samples (bounded by appropriate marker)
TOP10 (M1)I20259 (M1)I20269 (M2)I20270 (M2)I20268 (M2)I20260 (M2)
1.621.6634.6278.93133.87170.4
1.651.6833.4477.06133.29168.55
1.651.6734.1577.18137.02171.03
  • Calculated fluorescence intensities in consensus BioBrick™ GFP units (I/II20260) using (<Isample,MX>-<ITOP10,M1>)/(<II20260,M2>-<ITOP10,M1>)
TOP10I20259I20269I20270I20268I20260
00.0001781970.1926305790.4519265040.7905199381

DDM promoter chart.jpg