User:Dileep D. Monie/Notebook/2008-3-26

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  • Diluted 50 μl of overnight culture into 5 ml of pre-warmed (37°C) fresh media (1:100)
  • Grew for 4 h at 37°C with shaking at 70 rpm
  • Measured A600 of cultures in a 96-well plate (200 μl/well)
    • A600 are at background levels (cultures should be at an OD600 of 0.2 - 0.4 after 4 h)
    • Noted mistake in Supplemented M9 Media recipe and discontinued experiment