User:Dhea Patel/Notebook/Phosphorylation/2012/06/06

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Objective

Learn how to maintain an OpenWetWare Notebook.

Description

  1. Add experimental record here. Include what, how, and why...

Data

Fractions 4-6

     Ru complex+paper: 0.4826g
          Weigh Paper: 0.2662g
           Ru complex: 0.2164g

Fractions 7

     Ru complex+paper: 0.3234g
          Weigh Paper: 0.2612g
           Ru complex: 0.0622g

Fractions and Observations

  Fraction 4-6: Ru comp: 0.2164g | filtered clear liquid
    Fraction 7: Ru comp: 0.0622g | filtered clear liquid
    Fraction 8: Ru comp: 0.0028g | filtered yellow liquid
    Fraction 1: Ru comp: 0.0213g | filtered yellow liquid
    Fraction 2: Ru comp: 0.0219g |
    Fraction 3: Ru comp: 0.0011g |
    Fraction 9: Ru comp: 0.0004g |
   Fraction 10: Ru comp: 0.0039g |

Calculations

0.2164g ÷ (898.65g/mol) = 2.5E-4 mol --> 0.25 mmol Ru complex.

0.25 mmol Ru complex ÷ 5 equivalents = 0.05 mmol ATP

ATMP solution: 0.18 mmol × 5 equivalents = 0.9 mmol --> 1 mmol

5E-5 mol ATP × 507.18 g/mol = 0.02445 g ATP


Predicting product mass

RuComplex: RuC32H25N7 P2F12

ATP: C10H12N5 O12P3

RuATP: RuC42H37N12O12P5F12


Ru - 101.17 g/mol
  C - 504.42 g/mol
  H - 37.37  g/mol
  N - 168.12 g/mol
  O - 192    g/mol
  P - 154.85 g/mol
  F - 228    g/mol
Total - 1385.83 g/mol

Notes

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