Mb rxns at 25°C
- Stock solutions:
- Mb Citrate
- 1.6mg Citrate Mb was added to 1.0mL MeOH in a centrifuge tube.
- OPD
- 2.7 mg OPD in 2.7mL MeOH
- H2O2
- 0.114mL 30% H2O2 in 0.887mL MeOH
- Experimental Run
- Sample chamber set to 25C and to stir
- 2.025mL OPD stock solution
- 0.75mL H2O2 stock solution
- Start Data Collection
- 1ms Integration
- 10 scan average
- Wait 1 minute after each scan
- Stop after 2 hours
- Files saved as: Me_OPD_H2O2_Phosphate_MeOH_5_SCANNUMBER.txt
- 0.225mL Mb/MeOH added just before the third scan
- This is denoted in the data file for the third scan
Paul will make 1L phosphate buffer @ same pH as last week. He will also make 1L of phosphate buffer with 1 molar NaCl.
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