User:Dhea Patel/Notebook/Hemoglobin Project/2013/02/26

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Objective

  • to run UV-vis on 11.6mg p-sorbitol in Citrate Buffer in MeOH at temperatures ranging from 5°C to 50°C, with 5°C increments.

Protocol

  • MeOH blank was run in UV-vis and Fluorescence.
  • 11.6mg of myoglobin with KCl in 20mL 0.01M pH 3 citrate buffer (evaporated) made January 30th was run in UV-vis and Fluorescence.
  • The data was analyzed and compared to the data from January 30th. To find that the UV-vis absorbance was the same, but the Fluorescence had a greater intensity.
  • UV-vis from 02/26/2013 and 01/30/2013:

UV-vis of 11.6mg Citrate Solid in 1mL MeOH, taken February 26, 2013.png UV-vis of 11.6mg Citrate Solid in 1mL MeOH.png

  • Fluorescence from 02/26/2013 and 01/30/2013:

Fluorescence of 11.6mg Citrate Solid in 1mL MeOH, taken February 26, 2013.png Fluorescence of 11.6mg Citrate Solid in 1mL MeOH.png