User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/11/04

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Objective

  • to express ADA in LB media
  • to extract cells from media

Description

Please refer to [Kay's entry] for details and protocol for:

  • centrifuging the cells,
  • preparing IPTG,
  • preparing ADA protein expression culture,
  • Inoculating the cells,
  • Centrifuging of the cells (again),
  • Resuspention of cell pellets in binding buffer,
  • and storing the resuspended cells.