User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/11/03

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Objective

  • to amplify ADA protein in LB media

Description

Please refer to Kay's entry for the protocol and details of:

  • creating 4 sets of 35mL and 4 sets of 1L LB media,
  • autoclaving the media,
  • inoculating the media,
  • adding Kanamycin,
  • and creating the starter culture in the four 35mL LB media.