User:David K. Barclay/Notebook/Controlling Pancreas Cell Fate Using Transcription Factors/2015/02/26

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RT-PCR

  • Testing primers DB07-DB13 against cDNA06
  • Followed Sybr Green RT-PCR Protocol
  • 7 Primers (primer and template master mixes different)

Pancreas Cell

  • Passage by cell split protocol
  • Cells at 70% confluency in T-25 starter medium
  • Deactivate 2mL of trypsin with 4mL of DMEM/20% FBS (6mL cells)
  • Transfer 3mL of cells into 7mL of DMEM/20% FBS (2 T75 flasks)
  • Two T75 flasks of TC1-alpha Pancreatic cells in 37 incubator