User:David K. Barclay/Notebook/Controlling Pancreas Cell Fate Using Transcription Factors/2014/11/18

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Pancreatic Cell Culture

  • Insert content here...

RT-PCR

  • Protocol
  • Note on bench to make 750nM primers

ELISA

Data Analysis=

  • Experiment showed no concentration of Insulin or Glucagon (low absorbance)
  • Control experiment (only substrate) turned very blue (high absorbance)

           Added TBST in last washing step - activation of substrate?

Bioinformatics

GO Term Analysis

  • Should go over GO Terms again
  • G-protein linked to secretion, acceptance of glucagon/insulin (importance)
  • What GO Terms should we see?

           GSIS, Secretion, Packaging, Membrane Permeation Integral Proteins...