User:David K. Barclay/Notebook/Controlling Pancreas Cell Fate Using Transcription Factors/2014/11/15

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ELISA (Test)

  • Running through ELISA testing once to check for antibody viability as well as practice.
  • ELISA protocol found here
  • Math to find protein concentrations: Z (ug/mL) x V (uL) = 20 (ug/mL) x 100 (uL)
  • Solve for V with Z being concentration of protein found by Bradford
  • Stopped at NAP Blocking stage (can stop here, no such thing as overblocking)

Pancreatic Cell Culture

  • Cells at seeming confluence (~50%)
  • Passaged with 20% FBS DMEM into 10 cm^2 plate