User:David K. Barclay/Notebook/Controlling Pancreas Cell Fate Using Transcription Factors/2014/11/13

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Pancreas Cell Culture

  • Day 8. Changed cell media in t-25.
  1. Aspirate off current media
  2. Add 4mL (37 C) 10% FBS DMEM
  • Should look into increasing FBS concentration to create better growth environment.
  • Quantify needed confluence to effectively split cells (maybe 1:5 or 1:4)