User:David K. Barclay/Notebook/Controlling Pancreas Cell Fate Using Transcription Factors/2014/09/19

Cell Fate Switch by Synthetic Transcription Factors	Main project page Previous entry      Next entry
Passage 1, TC1-Alpha (2) 95% confluent with well. Cells passaged from thawed (9/5). Split Cells into two 10cm dishes. Used Protocol: Splitting Cells Volumes changed: 2 mL PBS, 0.5 mL Trypsin, 3.5 mL DMEM. Total cell volume= 4mL 2mL cell + 8mL DMEM in 2 10cm dishes