User:David K. Barclay/Notebook/Controlling Pancreas Cell Fate Using Transcription Factors/2014/06/25

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RT-PCR E004

Target Genes

Gene dilution RTPCR number +/- PcTF
PDX1 1:10 A1-3 treated
PDX1 1:10 A4-7 untreated
MAFA 1:10 B1-3 treated
MAFA 1:10 B4-7 untreated
GLP1R 1:10 C1-3 treated
GLP1R 1:10 C4-7 untreated
PCSK1 1:10 D1-3 treated
PCSK1 1:10 D4-7 untreated
IAPP 1:10 E1-3 treated
IAPP 1:10 E4-7 untreated
KCNQ2 1:10 F1-3 treated
KCNQ2 1:10 F4-7 untreated
mCh 1:1000 G1-3 treated
mCh 1:1000 G4-7 untreated
ACTB 1:1000 H1-3 treated
ACTB 1:1000 H4-7 untreated
nTc --- A,B,C,D,E,F,G,H8-10 ---

Results

Gene dilution +/- PcTF Mean CP delta CP double delta CP
PDX1 1:10 treated 0 0 Invalid
PDX1 1:10 untreated 0 0 0
MAFA 1:10 treated 34.440 0.3350 0.3624
MAFA 1:10 untreated 34.936 0.9245 1
GLP1R 1:10 treated 40 7.10E-3 0.2568
GLP1R 1:10 untreated 40 2.76E-2 1
PCSK1 1:10 treated 0 Invalid Invalid
PCSK1 1:10 untreated 0 Invalid Invalid
IAPP 1:10 treated 38.363 2.21E-2 3.14E-2
IAPP 1:10 untreated 35.333 0.7022 1
KCNQ2 1:10 treated 31.621 2.363 0.5395
KCNQ2 1:10 untreated 32.6919 4.381 1
mCh 1:1000 treated 33.3063 0.7350 Invalid
mCh 1:1000 untreated 0 0 Invalid
ACTB 1:1000 treated 32.862 --- ---
ACTB 1:1000 untreated 34.823 --- ---