User:David K. Barclay/Notebook/Controlling Pancreas Cell Fate Using Transcription Factors/2013/12/18

From OpenWetWare
Jump to: navigation, search
Owwnotebook icon.png Cell Fate Switch by Synthetic Transcription Factors Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

12/18/13

READING LIST - Assigned by Dr. Haynes


PROTOCOLS

  1. Bioinformatics workflow or finding PcTF targets: will be added this week after I work out some bugs
  2. Transfection of plasmid DNA into human cells: http://openwetware.org/wiki/Haynes:TransfectionPlasmid_Lipo
  3. RNA miniprep: http://openwetware.org/wiki/Haynes:TRIzol_RNeasy
  4. cDNA synthesis: Notebook page from Carly's project
  5. RT-PCR set-up: http://openwetware.org/wiki/Haynes:UPLassay (I fixed the assay design center link)


RESEARCH LITERATURE
Goal: be able to explain how these papers relate to your project...

  1. Wang YH, Wei KY, Smolke CD. (2013) Synthetic Biology: Advancing the Design of Diverse Genetic Systems. Annu Rev Chem Biomol Eng. 4:69-102 - Recent comprehensive review on the field of synthetic biology
  2. Haynes KA, Silver PA. (2009) Eukaryotic systems broaden the scope of synthetic biology. J Cell Biol. 187:589-96
  3. Haynes KA, Silver PA. (2011) Synthetic reversal of epigenetic silencing. J. Biol. Chem. 286:27176-82 - Pay particular attention to where the protocols listed above appear in this paper (and if they do)
  4. Bramswig NC, Everett LJ, Schug J, Dorrell C, Liu C, Luo Y, Streeter PR, Naji A, Grompe M, Kaestner KH. (2013) Epigenomic plasticity enables human pancreatic α to β cell reprogramming. J Clin Invest. 123:1275-84 - Pay particular attention to genes that are up-regulated after alpha cells are treated with the HDAC inhibitors. Make a list of these genes and enter the list into your OWW notebook
  5. Moss LG. (2013) Creating new β cells: cellular transmutation by genomic alchemy. J Clin Invest. 123:1007-10


Note: You can download these papers for free if you log in through http://www-ncbi-nlm-nih-gov.ezproxy1.lib.asu.edu/pubmed?otool=azuazslib