User:David K. Barclay/Notebook/Chromatin State Sensor/2015/07/01

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David Barclay 07/01

Passaging - U2OS

  • Protocol: Splitting Cells
  • Cell culture medium is: McCoy's 5A, 10% FBS (tet-free), 1% P/S
  • Use 90-100% confluent T-75
  • Make 1 new T-75 at a 1:10 dilution
  • Passage number: 2