User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2013/08/06

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Remains of the Day

  • Redo California strawberry oomycete PCRs, with lower annealing temperature (55 degrees) and more cycles - 50 perhaps vs 44. Also used the Invitrogen PCR buffer this time, instead of NEB which I used last time.
  • Start treating Bonny Best tomato leaves with TBG products at previously ascertained concentrations.
    • 1. Water control: deionized water was sprayed onto leaves until dripping
    • 2. Actigard powder was mixed at 100 ug / mL and sprayed onto leaves until dripping,
    • 3. Fraction F4, at a 1:75 dilution, was pH adjusted to 7, then sprayed onto leaves until dripping
    • 4. Fraction FX, at a 1:75 dilution, was pH adjusted to 7, then sprayed onto leaves until dripping
    • 5. TBG liquid, at a 1:50 dilution, was sprayed onto leaves until dripping
  • Put together some info for the Earth Alive project - strawberry and trees