User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2013/03/27

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Remains of the Day

  • Discovered that the Heinz 2401 which I've been planting may be TSH04 from Tomatoe Solutions of Chatham. Its the same variety used in the pyrosequencing study.
    • Product Name: TOMATO HYBRID TSH04 - SEED

TSH04 (99 days or 2260 heat units)is an early jointless processing hybrid with excellent yield for its maturity classification. Fruit size is very uniform, peeled colour is good and fruit peel cleanly. For product use, TSH04 has medium viscosity and good soluble solids. Vine size is moderate. Field holdability and resistance to blossom end rot is excellent. Overall, the combination of good fruit size and firmness coupled with good peeled colour increases peeled recovery. It is also being used successfully in organic tomato procuction. Many fresh market growers also use this hybrid as an early hand pick Roma style variety, to get into the fresh market earlier. TSH04 is resistant to Verticillium and Fusarium, and is tolerant to bacterial speck. Tolerant to Pinnacle herbicide. TSH18 and TSH04 are extensively used in the processing industry in the Mid-West U.S.A. and southern Canada to start the processing season, due to their earliness and general suitability for processing as either paste or peeled wholepack tomatoes. Organic tomato production: Several of our hybrids such as TSH04, TSH18 and TSH28 are used for organic tomato production because of their ability to produce well and to resist disease without the use of fungicides. (http://www.ic.gc.ca/app/ccc/srch/nvgt.do?lang=eng&prtl=1&sbPrtl=&estblmntNo=123456241469&profile=cmpltPrfl&profileId=1921&app=sold)

  • Planted 60 seeds of this TSH04 to assay the isolates of bacterial spot and speck - gonna grow them in trays with covers on the bottom shelf of growth room (used Sunshine mix 2 with Miracle Gro at 0.5 g/L added to wet the soil).
  • Growing overnights of the bacteria to make glycerol stock with - also thinking of making the best disease causers GFP tagged so we can visualize changes to bacterial populations in real time.
  • Transformed ligations of A tailed ITS4 terminal fragments (Root 150, 225 and 250/Rhizosphere 200, 250, 600 bp) into 30 ul of commercial competent JM109 E. coli