User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2013/02/21

Applied Soil Microbial Ecology

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Remains of the Day Bacterial PCR of the root samples from TVD growth room rep 2 failed again - just gonna go ahead and use the stuff from the first round and accept that they failed Did restriction of all the bacterial (HhaI) and fungal (BstNI) PCR samples for TRFLP. Did not purify the reactions. In the morning, I put tissue cultured Kennebec potatoes into 1:100, 1:250, and 1:500 dilutions (1 mL of which was added to 9 mL of PNCM, then autoclaved) of TBG liquid and FX fraction. Wait for a month to assay growth responses with photos and weights.