User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2012/10/02

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Remains of the Day

  • Screened the PCR I did for fungal TRFLP of Leamington and Tilbury tomato samples and also did successful test of the new Oomycete primers with dying arrugula grown on Holland marsh soil (see below)
  • Gel extracted the faint Oomycete bands and am ligating them so I can sequence
  • Redid faint fungal TRFLP amplicons for the TRFLP
  • Zymo extracted TVD soil, pasteurized and sand rhizosphere bacterial PCR at 56 and 48 degrees so we can compare effect of temperature and zymo extraction