User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2012/07/31

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Remains of the Day

  • Do colony PCR with M13 primers and restriction with DdeI of colonies from yesterday (although the TVD rhizosphere clones almost didn't grow at all!). 34 cycles with 58 degree anealing, 35 cycles, and 25 ul total volume.
  • Design primers for NifH TRFLP
  • Read and write review but focus today on the section for fungus biocontrol in production systems of considerable importance and available commercially