User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2012/01/06

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Remains of the Day

  • Redo qPCR of PR1 in potato and tomato
  • Call Norgen about tubes in soil DNA extraction kits (they are gonna look into giving us new ones)
  • Talk to George about PCR results and agree about TRFLP method and agreement meeting
  • Look at data and see about explaining outlier in Brazil trial
  • New bacterial primers use a specific recipe (I find these concentrations/volumes/times weird so discuss):

dH2O - 37.5 ul

10X Buffer - 5 ul

MgCl2 (50 mM) - 2 ul

BSA (20 mg/mL) - 1.25 ul

dNTPs (4 mM?) - 1 ul

Primer 63F-Max550 (20 mM) - 1 ul

Primer 1389-6FAM (20 mM) - 1 ul

Taq Polymerase - 0.25 ul

Total - 50 ul

PCR program is 2 mins 95, 30 cycles (1 min 95, 1 min @60 degrees, 2 mins 72 degrees), 10 mins @ 72 dgrees