User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2011/12/08

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Remains of the Day

  • Found useful presentation about breeding for bacterial disease resistance in tomato delivered by J.W.Scott. Some good photos and examples, including reference to S. penneli variety LA0716 being highly resistant variety and a photo from the collection site.
  • Did PCR of five 200 mg samples of TBG 13 soil using Norgen Kit. Then did 40 cycles of PCR with 48 degree annealing, using 1.5 ul of DNA in 25 ul PCR reaction (standard mix). As you can see below, genomic DNA was there, but no amplification occured, likely because of inhibitors. Gonna retry samples 1 and 2 using 50 ul volume, 0.5 ul of BSA, 2x diluted DNA, or 10 diluted DNA.