User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2011/11/29

From OpenWetWare
Jump to: navigation, search
Owwnotebook icon.png Applied Soil Microbial Ecology Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

Remains of the Day

  • Arrange with Steve Evans for more liquid nitrogen (maybe get to extract RNA today if you do)
  • Do nested PCR on NSA3/NCL2 primer PCRs from yesterday, using ITS1F and ITS4 (doing 35 cycles of gradient with 1ul of PCR product as template)
  • Do 799F and 1392R PCR on SantaMaria soil samples (spec them for concentration first)