User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2011/11/28

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Remains of the Day

  • Harvest potatoe leaf tissue with liquid nitrogen
  • Test nested primers out for fungal amplification (gradient PCR)
  • Figure out tomato genotypes to use in experiment: tomato genome is based on Solanum lycopersicum cv. Heinz 1706 (ACC # at UCDavis is LA4345), Beaufort is a famous root grafting variety (http://www.johnnyseeds.com/p-7472-beaufort-f1.aspx), RST-04-105 is a very disease resistant variety (http://www.dpseeds.com/content/rst-04-105-t-tomato), wild Peruvian tomato with 100% tomato like genes and disease resistance: LA1286, and then there's the suscetible Heinz variety we'll work with here too. A S. habrochaites variety referenced as resistant to root rot from Phytophthera is: LA407 - http://www.ncbi.nlm.nih.gov/pubmed/20465418 .

File:Nov28,2011Gel.tif