User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2011/11/10

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Remains of the Day

  • So either I am amplifying RNA or contaminating DNA with EF1, or it could also possibly be degraded RNA posing as PCR product. I will redo PCRs today using PR1 primers, and include RNAse treated cDNA as a treatment, along with DNAse treated RNA. Discussed with George, and most likely solution is extending DNAse digestion of RNA before making cDNA. Will do that tommorow, remake cDNA and test out later for DNAse contamination/PR1 gene expression.


  • Finishing TAQ production today
  • Doing journal club on "Deciphering the Rhizosphere Microbiome..."
  • Continue analyzing EtOH vs Kit TRFLP results