User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2011/11/03

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The Daily Grind

  • So, my Canadian and Mexican soil samples did not generate any data in the TRFLP. I hypothesize that the problem was too many cycles of PCR (70x), since sterile sand had very low titres and yet gave good TRFLP results with several clear peaks. Will redo 3 reps each of sand, can, mex and then split these PCRs into two to test the difference between ethanol precipitation and PCR purification kits (20 ul each).