User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2011/11/02

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The Daily Grind

  • At the end of the day, do 27F and 1492R PCR on Brazil samples in 96 well plate
  • Got TRFLP results from soil swap trial - they worked well, except Mexican and Canadian soils appeared to have less bacteria than sterilized sand! Probably an artifact of having too much DNA? Anyway, this seems to suggest that ethanol precipitation will work well for cleanup of fluorescently labelled PCRs.