User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2011/11/01

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The Daily Grind

  • Got this advice regarding the taq clone: -Grow 2mL culture in LB broth with ampicillin. Incubate over night (16hours) at 37C. Use 500uL of culture to inoculate 2 flasks with 1L of LB broth with ampicillin. Grow for 11 hours at 37C. Add 125mg/L of IPTG and incubate for another 12 hours at 37C.
  • Treated potatoes with spray (til wet) and/or drench (10 mL) of dH20, IBR, Actigard today. (30 ug/mL actigard, 1/100 IBR)