User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2011/07/04

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The Daily Grind

  • Gonna design/order primers today for DGGE - guess I should talk to Bea first.
  • Thinking of using 799f/1492r for 16S (nested from another gene product with primers from here), degenereate primers for nifH (PolFI/AQER-GC30 as found in this publication), and degenerate primers DegACC5′/DegACC3′ for ACC Deaminase based on this publication.
  • PolFI = TGC GAI CCS AAI GCI GAC TC
  • AQER-GC30 = CGC CCG CCG CGC CCC GCG CCC GGC CCG CCC GAC GAT GTA GAT YTC CTG
  • DegACC5′ = GGB GGV AAY AAR MYV MGS AAG CTY GA
  • DegACC3′ = TTD CCH KYR TAN ACB GGR TC