User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2010/06/24

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The Daily Grind

  • Redoing sequencing of 3A9, 3A11, 3B4, 3B11, 3C1, 3C2, 3C3, 3C10, 3D2, 3E1, 3E2, 3E3, 3E6, 3E7, 3E11, 3F1, 3F6, 3F8, 3F9, 3F10, 3G9, 3G10, 3G12, 3H3, 3H6, 3H10, 3H11
  • Making a redo of positive antibiosis colonies from plate 3,4,5. For 3 this means 3A6, 3B11, 3C6, 3C8, 3D3, 3d5, 3E8, 3E9, 3F8, 3G5, 3H5. Plate 4/5 will have (Bacillus) 4c11, 4c12, 4d1, 4d2, 4f10, 4G12, 5a1, 5a9, 5b9 (E. coli) 4B10, 4E11, 4G7, 4G9, 5A6, 5A7, 5A8, 5A10, 5A12

Took OD600 readings of bacteria grown in M9/LGI with arabinose and IPTG. The ACC deaminase containing E. coli trend to have higher ODs.

ACCOD.jpg

Without ACC With ACC L:ACC 1 0.403 0.666 L:ACC 2 0.222 0.555 A:ACC 1 0.301 0.471 A:ACC 2 0.322 0.533 A:ACCTerm 1 0.388 0.566 A:ACCTerm 2 0.247 0.461 A:GFP 1 0.444 0.323 A:GFP 2 0.272 0.501