User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2010/06/14

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The Daily Grind

  • To do: Harvest plasmid from overnights and test digests for inserts.
  • To do: Cut up potatoes for second round test dip (include another round of transgenic JM109 but at OD=0.2 instead of 1)
  • To do: Continue cleaning sequences for soil endophyte ID
  • To do: Do replica plating with 3 and redo 4/5 auxin, E.coli, Bacilus, Yeast, Siderophores

Actually did the replica plating, so letting them grow for 48 hours, followed by 24 at 4 degrees and then analysis