User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2010/05/27

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The Daily Grind

  • Did rep number two of the transgenic E. coli on potatoes assay.
  • Kp342, 1A1, 1A8, and 1C4 all appear to host pDRIVE, but none of them are expressing GFP off the Arabad promoter (1 ul/mL 10% Arabinose on plate), so I want to retry making the plasmids using the PSBA promoter which I know works (but which may cause cell death driving RNAi expression). Lets see if it works. If not, will have to try something else like T7. (actuallty, gonna quickly retry Arabinose induction of GFP using much higher amounts of Arabinose - 10 ul/mL)