User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2010/05/25

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The Daily Grind

  • Started a greenhouse trial with 1A1 (Pantoea agglomerans from B73) and 2A10 (Burkholderia phytofirmans from Mixteco). Germinated 20 seeds of Mixteco and Pioneer 3751 several days ago, and today dipped them in bacteria adjusted to OD600=0.2 in R2A. Negative controls were dipped in bacteria free R2A. All were put in turface in small pots and are being automatically watered in Amelie's greenhouse with her hoaglands solution (ask her for recipe and growth conditions). Will water each plant with 100 mL of the appropriate bacteria adjusted to OD600=0.2 every week on Tuesday for two months until plants are harvested and weighed.