User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2010/04/01

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The Daily Grind

  • Replating soil endophytes out using glycerol stocks. Taking 10 ul of glycerol (800 ul of juice plus 500 ul of 80% glycerol) and resuspending in 290 ul of phosphate buffer (plus cycolhexamide). Spreading 50 ul of that dilution on R2A plates.
  • Cut potatoes for BAC survival study.
  • Plated ligations of AraBad, Medium Promoter, and Weak Promoter to pSB1A2