User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2010/03/30

From OpenWetWare
Jump to navigationJump to search
Maize Endophyte Biofertilizers Main project page
Previous entry      Next entry

The Daily Grind

  • Got primers, so doing PCR of AraBAD promoter (cut and ligate with Xba/Spe into pSB1A2), medium promoter, weak promoter (cut them and pSB1A2 with Eco/Spe and ligate them all together). Then cutting the resulting plasmids with Nde/Pst, ligating in the GFPuv from pDSK-GFPuv. Plasmids with fluorescence will be used to recieve ACC, PDS RNAi, TB1 RNAi, and alsS (also cut with NdeI/Spe). The alsS plasmid will then need alsD put in afterwards using Spe plus dephosphorylation.
  • pouring plates for streaking out soil endopyte glycerol stocks (old plates were over grown with fungus)



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:David_Johnston_Monje/Notebook/Maize_Endophyte_Biofertilizers/2010/03/30&oldid=1003808"