User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2009/11/19

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The Daily Grind

  • To Do
    • Cutting PDS (Eco/Spe) in Maize Intron in pSB1A2 with Nde/Pst to check size of inserts (also cutting all with SpeI to recieve other frag)
    • Looks like E. coli ACC deaminase and alsSD functional screens are not working, so will do PCR screen on colonies now instead. Use purified pSB1A2 plasmids as positive PCR controls too (both VF/VR and ACC or alsSD specific primers). Save PCR amplified stuff for more cloning if I need it to.
    • Do PCR of PDS in pDRIVE colonies (3) and make overnights of the same ones for glycerol and plasmid extraction