User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2009/07/30

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The Daily Grind

  • Jacqui is doing 24 colony PCRs each of Ja, Tu, Bo, and Na from the 16S clone library.
  • At 12:30 going to do dilution plates of the 24 hour competition test. Have the same for tommorow (48 hours) and 72 hours. Will do 1000x and 10000x dilutions on R2A agar.
  • Trying a new thing out on my BAC library. First grew cultures up overnight with lots of chloramphenicol. Then added 100 ul of autoinducer solution (to 3 mL of culture) and grew another night. Then today am going to take 1 mL aliquots, pellet the cells, and resuspend in 1 mL of either LB, SOC, or TSB and grow overnight (half of the 1mL will be grown in sealed eppendorfs to see if anaerobic conditions might help). Tommorrow will assay for transgenic smell, and put on gnotobiotic potatoes to see if a difference was had.
  • Also transformed "positive" plasmids into DH5 alpha to see if I can replicate the acetoin production effect. Will have to make more broth before I can culture these overnight.