User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2009/05/28

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The Daily Grind

  • Going to troubleshoot cultures a bit : toothpicking current LGI microbes onto new LGI plate (eliminate the media carryover). Also going to toothpick a subset onto R2A plate and compare to old streak plates (also toothpicked onto the R2A plate). These will be turned into liquid cultures and added to new 96 well glycerol stocks of the endophytes
  • Planning to : mix together 16S PCR from all seeds and clone it, then do colony PCR and ARDRA with DdeI for clone sequencing
  • Goin to Waterloo for iGEM Canada meeting tomorow