User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2009/05/12

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The Daily Grind

  • Sarah made electrocompetent seed endophytes (about 15) and electroporated them with pDSK-GFPuv as per the E. coli instructions. 1mm cuvettes and program Ec1 on the Biorad electroporator.
  • Transformed ligations of partially NdeI cut alSD operon and NdeI cut ACC deaminase into (dephosphorylated) NdeI cut pSBA12 with PSBA::GFPuv and NdeI cut pDSK-GFPuv