User:David Benjamin Nyer/Notebook/PcTF breast cancer/2016/06/23

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Summary

Polymer transfection results: none of the 12 samples show high levels of fluorescence. Transfection efficiency appears to be very low regardless of polymer type or concentration. Massive amount of cell death in higher polymer concentration samples. Images were taken 24 hours post transfection.

AGC18 (1:5)

10:1 polymer:DNA
2016-06-23 MCF10A Well 1 AGC18 1-5 KAH126-MV2 10 1 phase mCherry 10x.jpg

25:1 polymer:DNA
2016-06-23 MCF10A Well 2 AGC18 1-5 KAH126-MV2 25 1 phase mCherry 10x.jpg

50:1 polymer:DNA
2016-06-23 MCF10A Well 3 AGC18 1-5 KAH126-MV2 50 1 phase mCherry 10x.jpg

NGC18 (1:5)

10:1 polymer:DNA
2016-06-23 MCF10A Well 4 NGC18 1-5 KAH126-MV2 10 1 phase mCherry 10x.jpg

25:1 polymer:DNA
2016-06-23 MCF10A Well 5 NGC18 1-5 KAH126-MV2 25 1 phase mCherry 10x.jpg

50:1 polymer:DNA
2016-06-23 MCF10A Well 6 NGC18 1-5 KAH126-MV2 50 1 phase mCherry 10x.jpg

NR

10:1 polymer:DNA
2016-06-23 MCF10A Well 7 NR KAH126-MV2 10 1 phase mCherry 10x.jpg

25:1 polymer:DNA
2016-06-23 MCF10A Well 8 NR KAH126-MV2 25 1 phase mCherry 10x.jpg

50:1 polymer:DNA
2016-06-23 MCF10A Well 9 NR KAH126-MV2 50 1 phase mCherry 10x.jpg

PGC18 (1:5)

10:1 polymer:DNA
2016-06-23 MCF10A Well 10 PGC18 1-5 KAH126-MV2 10 1 phase mCherry 10x.jpg

25:1 polymer:DNA
2016-06-23 MCF10A Well 11 PGC18 1-5 KAH126-MV2 25 1 phase mCherry 10x.jpg

50:1 polymer:DNA
2016-06-23 MCF10A Well 12 PGC18 1-5 KAH126-MV2 50 1 phase mCherry 10x.jpg