User:David Benjamin Nyer/Notebook/PcTF breast cancer/2016/06/01

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Summary

Performed RNA extraction using the RNeasy kit. Measured RNA concentration on MCF7 samples as well as the older BT474 & BT549 samples.

RNA Concentration

Cell Line Transfection Timepoint 260/280 ng/µL
BT474 no DNA 24 hr 2.07 558.898
BT474 KAH126-MV2 24 hr 2.053 278.071
BT474 KAH126-MV2 24 hr 2.052 378.685
BT474 no DNA 48 hr 2.058 604.635
BT474 KAH126-MV2 48 hr 2.041 211.429
BT474 KAH126-MV2 48 hr 2.056 273.118
BT474 no DNA 72 hr 2.056 825.997
BT474 KAH126-MV2 72 hr 2.044 334.982
BT474 KAH126-MV2 72 hr 2.044 294.869
BT549 no DNA 24 hr 2.046 235.827
BT549 KAH126-MV2 24 hr 2.022 102.534
BT549 KAH126-MV2 24 hr 2.039 141.041
BT549 no DNA 48 hr 2.042 191.945
BT549 KAH126-MV2 48 hr 2.003 72.571
BT549 KAH126-MV2 48 hr 2.029 126.021
BT549 no DNA 72 hr 2.05 354.439
BT549 KAH126-MV2 72 hr 1.936 98.815
BT549 KAH126-MV2 72 hr 2.038 147.312
MCF7 KAH126-MV2 24 hr 2.051 693.453
MCF7 KAH126-MV2 24 hr 2.046 408.767
MCF7 no DNA 24 hr 2.04 456.589
MCF7 KAH126-MV2 48 hr 2.056 346.299
MCF7 KAH126-MV2 48 hr 2.046 295.214
MCF7 no DNA 48 hr 2.051 331.211
MCF7 KAH126-MV2 72 hr 2.066 687.914
MCF7 KAH126-MV2 72 hr 2.052 420.697
MCF7 no DNA 72 hr 2.06 522.581