User:David Benjamin Nyer/Notebook/PcTF breast cancer/2016/05/27
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New attempt at transfecting MCF7 cell line with KAH126-MV2 plasmid.
Cells were transfected using standard lipofectamine LTX procedure, followed by centrifugation at 100 g for 5 minutes at room temperature. Cells were then returned to the incubator in antibiotic-free medium.