User:David Benjamin Nyer/Notebook/PcTF breast cancer/2016/05/27

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Summary

New attempt at transfecting MCF7 cell line with KAH126-MV2 plasmid.

  • Well 1: KAH126-MV2, 2.5 µg (24 hr timepoint)
  • Well 2: KAH126-MV2, 2.5 µg (24 hr timepoint)
  • Well 3: no DNA (24 hr timepoint)
  • Well 4: KAH126-MV2, 2.5 µg (48 hr timepoint)
  • Well 5: KAH126-MV2, 2.5 µg (48 hr timepoint)
  • Well 6: no DNA (48 hr timepoint)
  • Well 7: KAH126-MV2, 2.5 µg (72 hr timepoint)
  • Well 8: KAH126-MV2, 2.5 µg (72 hr timepoint)
  • Well 9: no DNA (72 hr timepoint)

Cells were transfected using standard lipofectamine LTX procedure, followed by centrifugation at 100 g for 5 minutes at room temperature. Cells were then returned to the incubator in antibiotic-free medium.