User:David Benjamin Nyer/Notebook/PcTF breast cancer/2015/11/25
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BT-474 transfection results and next steps.
BT-474 transfection results
Transfection efficiency very poor throughout, based on fluorescence microscopy. Not going to worry about harvesting and measuring efficiency via flow cytometry.
Next steps: set up two 6-well plates per cell type with 3 cultures per plate. Try these transfection optimization steps: