User:David Benjamin Nyer/Notebook/PcTF breast cancer/2015/11/23

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Summary

Transfecting BT-549 cells in 6-well plate using the optimized Lipofectamine LTX protocol, viewable here.

Methods

Used 2.5 µg of pMaxGFP plasmid per well.

Treatment plan:

Well no. pMaxGFP (µg) LTX Reagent (µL)
1 0 0
2 2.5 6
3 2.5 9
4 2.5 12
5 2.5 15
6 0 15

Wells 1 and 6 are negative controls. Well 1 is no DNA, no LTX. Well 6 is no DNA.

Cells are at 100% confluency, in 2 mL of BT-549 medium (no antibiotic). Overgrowth may impair transfection efficiency somewhat.