User:David Benjamin Nyer/Notebook/PcTF breast cancer/2015/11/23
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Transfecting BT-549 cells in 6-well plate using the optimized Lipofectamine LTX protocol, viewable here.
Used 2.5 µg of pMaxGFP plasmid per well.
Wells 1 and 6 are negative controls. Well 1 is no DNA, no LTX. Well 6 is no DNA.
Cells are at 100% confluency, in 2 mL of BT-549 medium (no antibiotic). Overgrowth may impair transfection efficiency somewhat.