User:David Benjamin Nyer/Notebook/PcTF breast cancer/2015/11/09

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Summary

Ideal neon transfection conditions for BT-474 and BT-549 cell lines (so far):

BT-474

Pulse voltage Pulse width Pulse no. Transfection Efficiency Cell Recovery (normalized to control)
1050 30 2 58.86% 32.86%
1300 10 3 43.27% 30.24%
950 30 2 42.12% 38.57%


BT-549

Pulse voltage Pulse width Pulse no. Transfection Efficiency Cell Recovery (normalized to control)
1400 20 1 35.08% 30.67%
1200 20 2 31.05% 26.99%
1200 30 1 29.48% 30.67%


Will use these values to create a list of transfection conditions for 12-well plates tomorrow.