User:David Benjamin Nyer/Notebook/PcTF breast cancer/2015/11/03

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Summary

Here are the neon transfection results:

2015-11-03 efficiency vs recovery.png

2015-11-03 efficiency vs voltage.png

2015-11-03 recovery vs voltage.png

There's a pretty clear correlation between increasing voltage and both increased transfection efficiency AND decreased cell recovery. Trick will be to find the sweet spot between the two extremes...


BT-474 data:

Sample Well no. Pulse voltage Pulse width Pulse no. Transfection Efficiency Live (cells/µL) Dead (cells/µL) Cell Viability Cell Viability (normalized to control)
1 A1 - - - 0.10% 420 5 98.82% 100.00%
2 A2 1400 20 1 31.91% 33 7 82.50% 7.86%
3 A3 1500 20 1 52.07% 12 12 50.00% 2.86%
4 A4 1600 20 1 67.65% 3 8 27.27% 0.71%
5 A5 1700 20 1 74.42% 9 16 36.00% 2.14%
6 A6 1100 30 1 27.72% 95 10 90.48% 22.62%
7 B1 1200 30 1 39.80% 49 8 85.96% 11.67%
8 B2 1300 30 1 43.37% 8 6 57.14% 1.90%
9 B3 1400 30 1 71.02% 18 7 72.00% 4.29%
10 B4 1000 40 1 29.46% 114 7 94.21% 27.14%
11 B5 1100 40 1 42.74% 36 7 83.72% 8.57%
12 B6 1200 40 1 68.75% 43 16 72.88% 10.24%
13 C1 1100 20 2 41.21% 81 7 92.05% 19.29%
14 C2 1200 20 2 58.26% 73 9 89.02% 17.38%
15 C3 1300 20 2 77.55% 20 4 83.33% 4.76%
16 C4 1400 20 2 87.93% 12 4 75.00% 2.86%
17 C5 850 30 2 18.07% 109 22 83.21% 25.95%
18 C6 950 30 2 42.12% 162 29 84.82% 38.57%
19 D1 1050 30 2 58.86% 138 14 90.79% 32.86%
20 D2 1150 30 2 71.43% 4 2 66.67% 0.95%
21 D3 1300 10 3 43.27% 127 55 69.78% 30.24%
22 D4 1400 10 3 53.64% 34 16 68.00% 8.10%
23 D5 1500 10 3 68.13% 52 12 81.25% 12.38%
24 D6 1600 10 3 75.87% 32 14 69.57% 7.62%

BT-549 data:

Sample Well no. Pulse voltage Pulse width Pulse no. Transfection Efficiency Live (cells/µL) Dead (cells/µL) Cell Viability Cell Viability (normalized to control)
1 A1 - - - 0.24% 163 25 86.70% 100.00%
2 A2 1400 20 1 35.08% 50 5 90.91% 30.67%
3 A3 1500 20 1 36.76% 19 3 86.36% 11.66%
4 A4 1600 20 1 43.33% 18 2 90.00% 11.04%
5 A5 1700 20 1 56.67% 3 1 75.00% 1.84%
6* A6 1100 30 1 0.48% 124 45 73.37% 76.07%
7 B1 1200 30 1 29.48% 50 9 84.75% 30.67%
8 B2 1300 30 1 37.29% 24 5 82.76% 14.72%
9 B3 1400 30 1 33.33% 2 1 66.67% 1.23%
10 B4 1000 40 1 19.64% 62 11 84.93% 38.04%
11 B5 1100 40 1 30.50% 34 5 87.18% 20.86%
12 B6 1200 40 1 41.74% 23 3 88.46% 14.11%
13 C1 1100 20 2 23.41% 55 16 77.46% 33.74%
14 C2 1200 20 2 31.05% 44 10 81.48% 26.99%
15 C3 1300 20 2 42.46% 18 3 85.71% 11.04%
16 C4 1400 20 2 44.58% 8 2 80.00% 4.91%
17 C5 850 30 2 12.60% 89 25 78.07% 54.60%
18 C6 950 30 2 26.27% 57 12 82.61% 34.97%
19 D1 1050 30 2 30.96% 24 7 77.42% 14.72%
20* D2 1150 30 2 0.64% 94 38 71.21% 57.67%
21 D3 1300 10 3 22.56% 39 7 84.78% 23.93%
22 D4 1400 10 3 29.59% 27 6 81.82% 16.56%
23 D5 1500 10 3 37.84% 11 5 68.75% 6.75%
24 D6 1600 10 3 50.65% 8 2 80.00% 4.91%