User:David Benjamin Nyer/Notebook/PcTF breast cancer/2015/10/20

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Summary

Transfected BT-549 with pMaxGFP in order to establish a baseline level of transfection efficiency.

Method

See the Mammalian cell transfection protocol. Three wells of a 6-well plate were seeded with BT-549 cells at approximately 2.5E5 cells/well density. Cultures were ~70% confluent at time of transfection. Plasmid used for transfection is pMaxGFP. Plasmid amount was 2 µg per well. Plate was returned to the incubator after transfection.

Next Steps

Tomorrow, measure transfection efficiency via fluorescence microscopy and flow cytometry.