User:Daniela A. Garcia S./Notebook/Modeling UNAM-Genomics Mexico/2010/08/04
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Wet Lab: OmpC/F promoter
OmpC transformation reporter
The OmpC tranformation from 03/08/10 was unsuccesful. It was got colonies just for the positive control BBa_I51020. The procedure would be done again, this time with 7μL of BBa_R0082.
2) 1' 42ºC
3) 5' on ice
4) 60' in 1 ml of LB medium at 37ºC
5) 1' at 13000 rpm
Two petri boxes were made with LBAm to plaque the transformed cells. 100 μL were added and left overnight at 37ºC.