User:Daniela A. Garcia S./Notebook/Modeling UNAM-Genomics Mexico/2010/08/03
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Wet Lab: OmpC/F promoterOmpC transformationThe part [BBa_R0082] (pOmpC) inserted in pSB1A2 was transformed in DH5α competent cells with the following protocol. The plasmid N.19 ([BBa_I51020]) was used as positive control.
2) 1' 42ºC 3) 5' on ice 4) 60' in 1 ml of LB medium at 37ºC 5) 1' at 13000 rpm
Electrophoresis Gel OmpF PCR_RTTHIn order to probe the PCRs reactions an electroforesis gel was made. This was loadded as follows:
NoteThe relation for all the antibiotics used is 1:1000 Modeling: BioBrick promotersFrom the article: "Measuring the activity of BioBrick promoters using an in vivo reference standard"
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