User:Daniela A. Garcia S./Notebook/Modeling UNAM-Genomics Mexico/2010/07/28
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Wet Lab: OmpF/C promoters characterization
Electrophoresis Gel OmpF PCR_RTTH
In order to probe the PCRs reactions an electroforesis gel was made. This was loadded as follows:
There were not positive results for none of the reactions, even for the B1T3 OmpF two bands were obtained.
A second set of PCR rections was made, this time with the TaqDNApol enzyme.
The temperature for extension (<<) was change. The one used for the RTTH PCR from 27/07/10 was to high, this cloud be a possible explanation why the reaction was unsuccesful.